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I am interested in validating the precence of a gene (FETUB) whose expression is in the lower quartile of the microarray (see figure in link).

https://drive.google.com/open?id=0B82aQne0d_mYWlFiMjRlb05CUVU

Do you think this gene would be detectable by PCR?

Thanks

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  • $\begingroup$ If your lab is lacking specific expertise on qPCR, and you need a sensitive and quantitative readout of transcripts, branched DNA single-molecule FISH may be less work than PCR ( while also being more sensitive and quantitative than RNAseq etc. ) $\endgroup$
    – tsttst
    Commented Jul 22, 2016 at 1:15

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If it was detectable in a microarray, the odds are very good for RT-rtPCR. If you are designing your own primers, make sure they span an exon junction, or if the gene is intronless, then span the UTR to exon junction to avoid amplifying DNA.

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