(Image Credits: Wikipedia) As it is clear from the image that we need both forward and reverse primers to get it working (Unless we have a sequence such that a single primer can work as both forward and reverse). I have read online here that including a single primer will result in linear amplification but I think there should be no amplification at all. Can you help me resolve my doubt?
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Using 1 primer will results in amplifying the original sequence in each cycle in a linear fashion: the primer will guide a single run of the polymerase. In opposed to the "chain reaction" in PCR which is exponential. Therefore, with one primer you linear amplification of say x40 times the original number of molecules you started with, whie with two primers you get exponential (~240) amplification. You right that with one primer, the amplification is neglectable, almost invisible.