I am trying to develop a method for figuring out how much of a certain mRNA is present in a bacterium. This is what I have so far:
- Perform a mRNA extraction
- Using Electrophoresis, to isolate the wanted mRNA strain (based off of size)
Now, I am not sure how I would quantify the amount of mRNA. I was thinking of using nano drop to test concentration, but I am not sure if that will give a good enough answer. What other methods could I use to quantify the amount of mRNA in my sample?