I am trying to estimate how much cardiomyocyte and its things (mitochondria) move in relation to neurons
- Movement of neurons (1)
- Movement of cardiomyocytes
- Movement of cardiomyocytes' mitochondrias (2)
- Movement of stem cells - I think we can ignore this one
Motivation: some imaging projects have focused on the neurons etc ECG/... because they move relatively little in comparison to cardiomyocytes and their organelles; my interest is in the electromuscular view and I would like to understand how the movement of cardiomyocytes and their mitochondrias could be adjusted/calibrated in the time-frequency (t-f) data (spectrogram/...) of cardiomyocytes
Simplistic view about including the effect of cytoarchitecture of organelles on the dynamics/movements based on statistics and probabilistic approaches
function getMitochondriaDynamics2D(signal2D, edges, Cytoarchitecture): signal2D=$1; edgesN=size($2,2); organelles=[neurons, cardiomyocyte_minus_mitochondia, mitochondria, ...]; % given organelles.cytoarchitecture = $3; % probabilistic values in Statistics organelles.dynamics = zeros(1,edgesN-1); % preallocation % Limit dynamics by cytoarchitectural restrictions for organelle in organelles % include here systemic effects on organelles but Ignored here % etc Age, Smoking, ... % Let's loop local changes for edge in organelles.dynamics organelle.dynamics(edge) = organelle.dynamics(edge) .* ... organelle.cytoarchitecture(edge); end end return signal2D .*organelle(mitochondria).dynamics; end %% TODO here function analysisMitochondriaDynamics2D(dynamics2D) ... end
To find those probabilistic values
- Embryology but I am weak here
- databases and/or publications - which?
I do not understand how much this should be considered here, but it could help to get any model
- Atrial cardiomyocyte calcium signalling
- mobility of the delayed-rectifer K+ channel Kv2.1 (3)
- cardiomyocyte adult mobility
Hypothesis and Math/Physics/Bio
Hypothetical View of t-f data (2D) in cardiomyocytes: regular points separated by steady-gel-like areas - - seems to artifact of the movement
Physics about characterizing such areas:
- ... [ which mathematical methods ] ...
There are much studies in Embryology about cardiomyocytes and their movement, but I cannot understand their relation in the topic
- Piquereau, Mitochondrial dynamics in the adult cardiomyocytes: which roles for a highly specialized cell?, 2013
- How to measure Na/K channel activation at the membrane level?
- Localization and mobility of the delayed-rectifer K+ channel Kv2.1 in adult cardiomyocytes.
- Mitochondrial dynamics in the adult cardiomyocytes: which roles for a highly specialized cell?
- PubMed with
cardiomyocyte+"patch clamp", about measuring plated cells which are from hearts freshly minced in collagenase / EDTA. See PMIDs 27364017, 27076034, 26142302, 26241168, 26378152, ... [NickSandor]
- adult cardiomyocyte
- possibly: Atrial cardiomyocyte calcium signalling
- Mitochondrial dynamics
- cytoarchitecture restrictions dynamics