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I am trying to estimate how much cardiomyocyte and its things (mitochondria) move in relation to neurons

  1. Movement of neurons (1)
  2. Movement of cardiomyocytes
  3. Movement of cardiomyocytes' mitochondrias (2)
  4. ...
  5. Movement of stem cells - I think we can ignore this one

Motivation: some imaging projects have focused on the neurons etc ECG/... because they move relatively little in comparison to cardiomyocytes and their organelles; my interest is in the electromuscular view and I would like to understand how the movement of cardiomyocytes and their mitochondrias could be adjusted/calibrated in the time-frequency (t-f) data (spectrogram/...) of cardiomyocytes

Pseudocode

Simplistic view about including the effect of cytoarchitecture of organelles on the dynamics/movements based on statistics and probabilistic approaches

function getMitochondriaDynamics2D(signal2D, edges, Cytoarchitecture):
signal2D=$1;
edgesN=size($2,2); 
organelles=[neurons, cardiomyocyte_minus_mitochondia, mitochondria, ...]; % given
organelles.cytoarchitecture = $3; % probabilistic values in Statistics
organelles.dynamics = zeros(1,edgesN-1); % preallocation 

% Limit dynamics by cytoarchitectural restrictions
for organelle in organelles
    % include here systemic effects on organelles but Ignored here
    % etc Age, Smoking, ... 

    % Let's loop local changes
    for edge in organelles.dynamics
        organelle.dynamics(edge) = organelle.dynamics(edge) .* ...
            organelle.cytoarchitecture(edge); 
    end
end   

return signal2D .*organelle(mitochondria).dynamics; 

end

%% TODO here  
function analysisMitochondriaDynamics2D(dynamics2D)
    ... 
end 

To find those probabilistic values

  • Embryology but I am weak here
  • databases and/or publications - which?

Biochemistry

I do not understand how much this should be considered here, but it could help to get any model

  • Atrial cardiomyocyte calcium signalling
  • mobility of the delayed-rectifer K+ channel Kv2.1 (3)

Useful keywoards

  • cardiomyocyte adult mobility

Hypothesis and Math/Physics/Bio

Hypothetical View of t-f data (2D) in cardiomyocytes: regular points separated by steady-gel-like areas - - seems to artifact of the movement

Physics about characterizing such areas:

  • ... [ which mathematical methods ] ...

Sources

There are much studies in Embryology about cardiomyocytes and their movement, but I cannot understand their relation in the topic

  1. Piquereau, Mitochondrial dynamics in the adult cardiomyocytes: which roles for a highly specialized cell?, 2013
  2. How to measure Na/K channel activation at the membrane level?
  3. Localization and mobility of the delayed-rectifer K+ channel Kv2.1 in adult cardiomyocytes.
  4. Mitochondrial dynamics in the adult cardiomyocytes: which roles for a highly specialized cell?
  5. PubMed with cardiomyocyte+"patch clamp", about measuring plated cells which are from hearts freshly minced in collagenase / EDTA. See PMIDs 27364017, 27076034, 26142302, 26241168, 26378152, ... [NickSandor]

Useful keywords

  • adult cardiomyocyte
  • possibly: Atrial cardiomyocyte calcium signalling
  • Mitochondrial dynamics
  • cytoarchitecture restrictions dynamics
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