So the thing is, I was getting a bacterial CRISPR kit for biology class, which is overall pretty simple and I'm just doing this for practice, and my dad was saying he was concerned it would accidentally make the non-pathogenic bacteria I was using pathogenic. I told him that it was basically impossible for me to accidentally genetically engineer bacteria to be pathogenic because infectious bacteria need a bunch of adhesins to infect the human body. However, that did make me think, what chances would there be of non-pathogenic bacteria (e coli hme63) becoming pathogenic because of some bacteria in the air getting in the culture and transferring plasmids to become infectious? Again, I don't think this is likely at all, but I would like to ask those with more knowledge than me. Edit: it would appear to me that if it was that easy for bacteria to get what they need to be infectious people would be getting sick a lot more than they are.