I am currently on the lookout for Trypsin, but I have some trouble finding something that is fairly cheap, ships to Europe and is equivalent to this Trypsin:

I've found Trypsin on Sigma:

However I am not sure how to compare the activity between the two types and ultimately which concentration I should then use in my experiment if I go with the Sigma one (the protocol I am following uses the EMS Trypsin in a 1g:100ml fashion). Can anyone shed some light on this? Or perhaps show me the way to some affordable Trypsin that can be shipped to Scandinavia?

Cheers! /Patricia

*The trypsin will be used for diaphonization (see comment)

  • $\begingroup$ Could you update your question to explicitly state what you will use the trypsin for. Trypsin has a number of uses and the use alters which trypsin is appropriate. $\endgroup$ – Michael_A Aug 24 '16 at 22:20
  • $\begingroup$ Hi Michael. Sure! I will use the trypsin for diaphonization (aka. clearing and staining), which is basically a way of dyeing a whole specimen. Trypsins role is to digest the specimen enough so it becomes transparent, but not so much that it falls apart... If that makes no sense there is a nice video about it here: youtube.com/watch?v=haopSRCuPdo... I have experience with use of Trypsin with cells - but diaphonization is completely new to me, and the protocols I have give no clue to what type of trypsin they are using. $\endgroup$ – Patricia Aug 25 '16 at 12:49

According to Sigma-Aldrich,

1 USP Unit = 3.0 BAEE Units 1 NF Unit = 1.1 USP Units

So 1 NF unit is roughly equal to 3.3 BAEE units. You can then directly compare the two trypsin powders, since they are preparations of the same enzyme.

| improve this answer | |
  • $\begingroup$ Yeah, I did see that, but the thing that bothered me is then that I need to compare unspecific units like >100 NF and 1,000-2,000 BAEE, and so I am left with questions like "How much is >100 NF? 101 NF? 500 NF?" and "Do I calculate with 1.000 or 2.000 BAEE?" $\endgroup$ – Patricia Aug 25 '16 at 12:52
  • $\begingroup$ But I guess the best solution is to note that 100 NF ~ 330 BAEE and so if I end up buying the Sigma one, I can start out with trying a 1:300 solution and see how that goes :) $\endgroup$ – Patricia Aug 25 '16 at 13:03

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