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Suppose I have got two sets of proteins (amino acid sequences). In one set, I find that the variance in the percentage concentration of the Cys is way more (65.34) than the variance of Cys in the other group (0.456). Does it mean anything? Does this have any sort of biological implication?

The concentration has been calculated by dividing the total number of Cys found by the size of the protein sequence.

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closed as unclear what you're asking by MattDMo, kmm, AliceD, James, rg255 Aug 29 '16 at 21:32

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    $\begingroup$ It may or may not mean anything. Without additional information no inference can be drawn. $\endgroup$ – WYSIWYG Aug 25 '16 at 10:21
  • $\begingroup$ What additional information is needed in this case? $\endgroup$ – girl101 Aug 26 '16 at 3:49
  • $\begingroup$ A lot: mean cysteine density, other structural and biochemical properties of the protein etc. Basically just the variance in cysteine content won't tell you anything. $\endgroup$ – WYSIWYG Aug 26 '16 at 8:15
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    $\begingroup$ This question would be more answerable with an introduction to the biological context, a description of how each dataset was generated (included the sample sizes), and the relative percentages of other amino acids. $\endgroup$ – James Aug 26 '16 at 10:06
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No, because it's extremely likely your sets are not a representative sample of the true distribution in proteins.

Your bias might be caused by an effect on the functionality of the protein, but it might as well be a bias in which organisms are sequenced or something caused by the alignment or the way you are searching for your sets.

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  • $\begingroup$ If there be is a hint towards functionality of protein, what is that functionality? Any sort of hint is what I want to know $\endgroup$ – girl101 Aug 26 '16 at 3:50
  • $\begingroup$ I'd say this is 99% biased searching / sequence collecting. $\endgroup$ – VonBeche Aug 26 '16 at 7:32
  • $\begingroup$ What would be the bias? What is the biological factor that creates this bias? $\endgroup$ – girl101 Aug 26 '16 at 7:36
  • $\begingroup$ "a bias in which organisms are sequenced or something caused by the alignment or the way you are searching for your sets" $\endgroup$ – VonBeche Aug 26 '16 at 7:38

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