I'm looking for the half-life of dNTPs, either as a whole or broken into individual bases, at 95 degrees C (or similar). A titration would be great if that exists. I can provide more specifics if need be, but broadly the idea would be 'able to polymerize' vs. 'unable'. Thanks in advance.

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    $\begingroup$ Interesting question. FWIW, I was able to get strong bands on a 6 hour long PCR reaction with Pfu polymerase, so it is unlikely that dNTP stability would be a very big concern under most circumstances. $\endgroup$ – March Ho Aug 30 '16 at 20:55
  • $\begingroup$ @MarchHo Thanks for the insight. To confirm, when you say 6 hour long PCR, that is the total length of the program, and not a total time of 6 hours at 95C (or 98C perhaps with Pfu)? $\endgroup$ – metaditch Aug 30 '16 at 21:11
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    $\begingroup$ The total time was 6 hours long, most of which was extension at 72C. I would say the time taken at 98C was roughly 25 minutes (30s per cycle) $\endgroup$ – March Ho Aug 30 '16 at 21:13
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    $\begingroup$ I think almost every molecular biologist must have wondered about this (as we always assume that (d)NTPs are unstable) but there seems to be no study that checks the stability of (d)NTPs at different temperatures. The closest I could find is this article which says that 40% of ATP survives after 1h at 95⁰C (10mM in distilled water). Mg²⁺ would increase the stability and stability of dATP may probably be higher. $\endgroup$ – WYSIWYG Aug 31 '16 at 6:02

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