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So, I have calculated the frequency of all SNPs (single nucleotide mutations) in some samples and have also found the nucleotides on the 3' and 5' sides of the mutated nucleotide. So I have a list of 192 possible permutations like this one:

AAA -> ACA 43
AAA -> AGA 35
AAA -> AAA 134
.
.
TGA -> TAA 345
TGA -> TCA 25
TGA -> TTA 97
.
.

What is the statistical approach to show that a particular set of 4 of these mutations is significant?

I have little knowledge in statistics so I welcome a for-dummies explanation!

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closed as unclear what you're asking by Remi.b, AliceD, rg255, James, fileunderwater Sep 26 '16 at 7:36

Please clarify your specific problem or add additional details to highlight exactly what you need. As it's currently written, it’s hard to tell exactly what you're asking. See the How to Ask page for help clarifying this question. If this question can be reworded to fit the rules in the help center, please edit the question.

  • $\begingroup$ Please do not suggest it is a good assumption to assume you are an idiot. It is wrong and you should work on knowing it better. I edited your post. $\endgroup$ – Remi.b Sep 19 '16 at 14:50
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    $\begingroup$ If you are wondering about statistical significance, it means that you want to do a statistical test. In order to make a test, you need a question to test. What is your question? Is it "Is a given SNP under selection?" or "Does the SNP really exist or is it just a sequencing error?" $\endgroup$ – Remi.b Sep 19 '16 at 14:53
  • $\begingroup$ Hi, the stupidity I was referring to was statistics-specific :) But, you are right. Anyway, I am trying to prove that the SNP is under selection. Specifically, that there is a higher frequency of TCA>TTA and TCT>TTT. $\endgroup$ – nikonan Sep 19 '16 at 19:20
  • $\begingroup$ Cool. You should clarify in your question that you are trying to detect selection. Also, what samples do you have exactly. Do you have different species or a single species? Do you have a structured population or a panmictic population? Do you have candidate genes (or SNPs)? $\endgroup$ – Remi.b Sep 19 '16 at 20:59