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In the figure, you can see that melting peaks differ by ~1°C at each sample.

Should I be worried?

enter image description here enter image description here

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  • $\begingroup$ I would have said that 1⁰C is not a significant difference but the technical replicates are so perfectly matching!! Are these samples from same source (tissue/cells etc)? $\endgroup$ – WYSIWYG Sep 22 '16 at 15:23
  • $\begingroup$ they are the same tissue (oviduct) from two different animals $\endgroup$ – Sergio.pv Sep 22 '16 at 15:27

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