A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B. This produces a 3000 bp and a 2000 bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp.
If the first site for enzyme A (A1) is present at the 100th base, the order in which the remaining sites (A2, B1 and B2) are present is -
(A) 3100, 5100, 8100
(B) 8100, 3100, 5100
(C) 5100, 3100, 8100
(D) 8100, 5100,. 3100
NOTE: This is a Kishore Vaigyanik Protsahan Yojana 2014 exam (stream SX) Question. I really couldn't understand the diagram shown in the solution, and my teachers were of no help either. The diagram in the solution is: