we do lot of gram staining and some spore staining. we normally wash our slides with detergent then rinse in D/w, dry it and store it. But on reusing this slide we have to flame it and also clean it with alcohol number of times to make it grease free. also these reused slides have some sort of deposit which does not go with cleaning and evend oes not hamper our staining too. can anyone please suggest the best simple way to clean microscopic slide and to make it grease free and how to store it.
An old technique to clean used slides is to just toss them in a jar containing equal volumes of distilled water, ethyl alcohol 95% and xylene. Shake from time to time. When needed wipe them dry using a clean, lint free cloth and use.
The techique was at the time (1930's?) in use in the lab of prof. Baudet (Veterinary parasitologist, Utrecht University) and is mentioned in several editions of the "Précis de Microscopie" by Maurice Langeron.
Langeron mentioned that the slides treated that way were "perfectly grease-free".
Another method is to wash the slides, rinse in distilled water and store for some time in a mixture of ethyl alcohol and acetic acid.
When on a tight budget, used Carnoy fixative was (is?) used.
This is a trick that was (is?) in use at some botanical labs in Eastern Europe.
It's mentioned in some of the German literature on microtechnique, if memory serves me right in Braune, Leman and Taubert's "Pflanzenanatomisches Praktikum".