I have a list of 100+ proteins and I need to conduct psi-blast for each one of them against "bacterial proteins" only.
I went to NCBI's protein blast tool, but couldn't figure out how to select/limit the targeted database.
Here are the 2 configs I tried:
Can anyone tell me if either of the approaches is correct? If not, please point the right way.
Edit 1 - add more details
All of my data is from "Supplementary Table 10" of paper "Comparative genomics of the neglected human malaria parasite Plasmodium vivax".
About 5% of all my protein sequences are ISS predicted. Remaining sequences are IEA predicted