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I have 6 vials of Pyrocystis lunula that I would like to grow. What would be a more suitable container, a tank or a clear jar? Also, I would like to know what kind of nutrient solution to use. Is it possible to get them to reproduce at a rate that eventually there can be a whole tank full of them?

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  • $\begingroup$ What sort of research have you done to try and answer your own question? $\endgroup$
    – MattDMo
    Commented Nov 14, 2016 at 3:07

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I have no experience with that particular species but dinoflagellates are often slow growing, very tricky to get to high densities and hard to maintain for a long time without proper experience and lab conditions.

If you still want to have a go I would recommend the following. Growth conditions are rather similar for most dinoflagellates: use a rotary shaker (gentle rotation) in a 12:12 day night regime, don't dilute to hard when subculturing and use standard F2 medium (see link for recipe). If you cannot get hold of seawater you can use saltwater aquarium salts such as Instant Ocean.

Most hate temperature changes so best to keep them at 18-21C

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  • $\begingroup$ So I can just go to the beach and get some sea water from their? $\endgroup$
    – LilVinny
    Commented Nov 15, 2016 at 1:19
  • $\begingroup$ You will have to sterilise it first (autoclave) and add nutrients such as iron, nitrate and phosphate. See the F2 recipe on the link provided $\endgroup$
    – mimat
    Commented Nov 15, 2016 at 14:48

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