One basic thing I'm not too clear on, which I would like to be clear on, is the brightness per mass unit of GFP or luciferase. I was having trouble finding a resource that would state the brightness at least for standard GFP or luciferase per gram, for example. Is there a basic rule for the maximum brightness for either of them? I'm aware this might end up being a vague question, but I could use help clarifying this. I found brightness as a product of the extinction coefficient and quantum yield but I wasn't sure how to translate that to watts.

  • $\begingroup$ Search quantum yield $\endgroup$ – WYSIWYG Nov 17 '16 at 5:22
  • $\begingroup$ So I understand that the quantum yield is the ratio of photons emitted to photons absorbed, but I still don't know what's generally the maximum brightness of at least certain kinds of GFP under a sufficiently bright light source, if that question makes sense. $\endgroup$ – Tom Nov 17 '16 at 14:11

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