1
$\begingroup$

I know that trypsin is used for proteomic studies, but I do not understand why trypsin digestion is necessary/important.

$\endgroup$

closed as unclear what you're asking by anongoodnurse, kmm, James, fileunderwater, AliceD Nov 22 '16 at 10:19

Please clarify your specific problem or add additional details to highlight exactly what you need. As it's currently written, it’s hard to tell exactly what you're asking. See the How to Ask page for help clarifying this question. If this question can be reworded to fit the rules in the help center, please edit the question.

  • $\begingroup$ Welcome to SE biology. Could you edit your question to make it clearer. Proteomics is not a synonym for mass spectrometry. I think you want to ask Why is tryptic digestion used prior to mass spectrometry? $\endgroup$ – Michael_A Nov 19 '16 at 0:23
0
$\begingroup$

Trypsin digestion allows you to cut proteins into smaller polypeptides, masses of which can be determined by mass spectrometry and then translated back into possible amino acid sequences. Trypsin cleaves after specific amino acids. Altogether, this allows you to identify proteins.

$\endgroup$

Not the answer you're looking for? Browse other questions tagged or ask your own question.