My task is to take photographs of fungus spores under a microscope at x100 using oil immersion.
My problem is that I get all these little blobs. How do I prevent the blobs?
So, I have been instructed to first put the spores on the glass slide, then add a teensy drop of water to it (I use a syringe so I have exact control over the droplet) and then put the coverslip on top of that. Then I tap the coverslip down with the eraser-end of a pencil to force the excess water out from under the coverslip. The immersion oil goes on top of that and then I can view the spores and take the photos.
This is what I mean:
All those little blobs aren't supposed to be there. I can't tell what are spores and what aren't, or if these spores have ornaments or not. However, I happen know this spore does have ornaments (rhodotus palmatus).