I need to isolate a protein that a bacteria has excreted into an agar plate. My plan is cut out parts of the agar, heat the agar to melt it and then concentrate the protein through some purification kit, and then run SDS-PAGE to see if I was successful in isolating the protein.
However, I am not sure whether heating the unknown protein before SDS-PAGE will effect the gel run in anyway. Does anyone know? I know if might denature the protein in some way, but I am doing that anyways with SDS.