I am generating stable cell lines and have something like 25+ culture flasks to take care of. Ideally I can let dead cells sit in the media with the live adherent without rushing to change media constantly - Will this affect growth of live cells - I don't want to hinder them as they are already sitting in high[] Hygromycin

  • $\begingroup$ I think, you should change the medium in a normal frequency, so every 2-3 days. But I don't think, that you have to change it every day. The cells shall die so in the end you have small populations which fit your purposes. $\endgroup$ – SeRe Nov 21 '16 at 20:27

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