I am working in biological waste water treatment and I need to collect some activated sludge samples and have them characterized through different methods. The characterization will take from a few days to a week. How do I keep the sludge viable in this time? How do I make sure the bacteria do not die?

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    $\begingroup$ What about cooling these samples to 4°C? $\endgroup$ – Chris Dec 12 '16 at 7:31
  • $\begingroup$ I though about that as well, but ... I do not know if it is enough. I was thinking about aerating them and feeding some... yeast extract now and then? I don't know. That;s why I am asking the experts. :D $\endgroup$ – Pompilia Dec 12 '16 at 7:41
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    $\begingroup$ @Pompilia activated sludge would have different kinds of microbes and the general yeast-extract based media may not be suitable for all the microbes. Therefore, I think 4°C cooling may be a better option. $\endgroup$ – WYSIWYG Dec 12 '16 at 8:18
  • $\begingroup$ @WYSIWYG It is also the simplest method, so I'm fine with that. Let's see if someone suggests something else. Thanks! $\endgroup$ – Pompilia Dec 12 '16 at 8:27
  • $\begingroup$ Have a look at this review: sciencedirect.com/science/book/9780123914538, it discusses the issue of mass-producing organisms in a more general sense (i.e. not just bacteria) $\endgroup$ – hello_there_andy Mar 20 '17 at 21:44

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