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See the image below for a visual representation of the 16S rRNA gene. It is composed of conserved and variable regions however I can't see to find a reasoning for why this is the case

enter image description here Thanks

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  • $\begingroup$ Are you talking about the variablility/conservation and structure of the RNA or of the gene (which would presumably include other parts of the DNA)? If it's just the rRNA, you should remove references to the gene, as this is confusing. (Also, it's "its", not "it's" for the possessive of it, the pronoun, and other pronouns except one.) $\endgroup$ – David Jan 2 '17 at 18:58
  • $\begingroup$ It would also be useful if you could indicate your source so that an answer can be addressed appropriately. What have you read about conserved or variable regions? $\endgroup$ – David Jan 2 '17 at 21:26
  • $\begingroup$ I added an image to try to be more specific to the question. I'm talking about the gene, but obviously that impacts the RNA/protein and everything else. $\endgroup$ – TheFoxx Jan 3 '17 at 9:20
  • $\begingroup$ Thanks: that helps. I used to follow the literature on rRNA structure and will dust off my papers (so to speak) and get back. I must say I have never seen a diagram like that — where did you find it? $\endgroup$ – David Jan 3 '17 at 10:36
  • $\begingroup$ OK. Found a related paper myself and answered the question. $\endgroup$ – David Jan 3 '17 at 23:10
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The structure of ribosomal RNAs involves double-helical stems, on the one hand, and loops or junctions, on the other. A comparison of small-subunit rRNAs of different sizes shows that, despite increasing complexity, a basic core structure exists. The is illustrated in the diagram below is adapted from Brimacombe, Trends in Biochemical Sciences (June 1984) 273–277.

Conserved features of 16S rRNA

From a structural (3D) point of view, both stems and loops are conserved, but as far as the nucleotide sequence is concerned, the helical stems are less conserved than the loops and junctions. This would appear to be because the main function of the helices is structural — so an A–U base pair can generally be replaced by a G–C (or U–A or C–G) base pair, whereas the loops are involved in interacting with various species — tRNA, initiation, elongation and termination factors, ribosomal proteins and the 50S subunit — which may involve more specific bases.

The main thrust of research on ribosomal structure (and what has interested me) is the relation of structure to function, but Carl Woese realized that, because of its ubiquity, rRNA was an excellent molecule for phylogenetic studies. Although, if one compares, say, mitochondrial ribosomes with mammalian ribosomes the sequence conservation is small, with different strains of bacteria it is much larger. This has led microbiologists interested in bacterial phylogeny to analyse the best regions for comparison in 16S rRNA, in terms of their degree of conservation, resulting in the concept of variable and conserved regions in the diagram in the question. (I find this terminology rather unfortunate because of its similarity to the totally unrelated immunoglobulins.) It appears that this is due to van de Peer et al. (1996), from whose paper I reproduce the figure below.

Conservation of regions of 16S rRNA

[Figure 3 of van de Peer *et al.*The most variable positions are in red, the most conserved in blue. Absolutely conserved positions are indicated in purple. Nucleotides present in E.coli but absent in >75% of the bacterial sequences considered are indicated in pink.]

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