I want to load my agarose gel with my PCR products, which are 50 µL reactions.

I am wondering how much of those reaction I can load into one well if I use a standard agarose mini gel (10 well comb, ~8 mm thickness, 1.2%).

Should I try loading the entire 50 µL into one well or should I split it into two?

Thanks in advance


  • $\begingroup$ Why do you want to load so much sample? $\endgroup$
    – Chris
    Jan 13, 2017 at 10:39
  • $\begingroup$ Low concentration or for gel extraction I would assume... $\endgroup$
    – Joe Healey
    Jan 13, 2017 at 11:03
  • $\begingroup$ Yeah, it is for gel extraction. I want to load maximum sample while using the minimal amount of gels $\endgroup$
    – Philz0rs
    Jan 13, 2017 at 11:30
  • $\begingroup$ If this is for gel extraction, load two lanes. Or connect two on the comb with tape to make a bigger well. $\endgroup$
    – Chris
    Jan 13, 2017 at 11:47

1 Answer 1


How many samples have you got? You can tape neighbouring teeth of the comb together to create double- or triple-wide wells to load more sample.

I don't normally load more that about 20 µL.

You could also make the gel thicker potentially.

  • $\begingroup$ Thanks for the tip! Unfortunately, I only have one standard agarose apparatus, and it doesn't allow me to use wider gels. I tested with loading buffer in one well, whether or not it would fit 50 µL. It did, but I didn't take the risk of losing sample due to leakage, so I will just run more gels. Thanks a lot, though! $\endgroup$
    – Philz0rs
    Jan 13, 2017 at 11:32
  • $\begingroup$ @Philz0rs If this answer addressed your problem, please consider accepting it by clicking on the check mark/tick to the left of the answer, turning it green. This marks the question as resolved to your satisfaction, and awards reputation both to you and the person who answered. If you have >= 15 reputation points, you may also upvote the answer if you wish. There is no obligation to do either. $\endgroup$
    – MattDMo
    Jan 13, 2017 at 12:58

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