I am no biochemist, but I want to purify some liters of solution through dialysis. What I know are those dialysis tubes in glas vessels on stir plates for labs. But how is this done in industry for bigger quantities in an affordable way? Background: The solution contains a precipitated enzyme (183 kDa) with ammonium sulfate (132 Da). I want to take away the ammonium sulfate to get back the active state of the enzyme.
Ammonium Sulfate (AS) precipitation is used to concentrate protein. After AS precipitation, you would centrifuge the sample to pellet the protein and then resuspend it in a small volume of water or buffer. You can then dialyze this small volume.
There are other methods of concentrating large volumes of protein, including chromatography and ultrafiltration.