All cells are seeded at the same concentration and all are harvested for analysis on the same day.
Cells were seeded on the same day. Cells were stimulated at 3:00 on a Wednesday (24 hour timepoint). Cells were then stimulated at 09:00 am on Thursday (6 hour timepoint) followed by 12:00 pm Thursday (3 hour timepoint) and so on until 3:00 pm on Thursday - at this time ALL cells were harvested and put into separate tubes according to what they were stimulated with and when e.g. 3 hour M-drug.
All tubes were analysed in sequence to measure the percentage of cells in that test tube that stained positive for that drug (i.e. as a means of measuring drug uptake)
Question: I have measured the percentage uptake of a drug at different time-points (0.5 - 24 hours) for the three drug variants. % uptake is taken as the % of cells that stain positive for that drug
What would the correct stat test be? Would a 3-way ANOVA make sense?