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How much tissue would be required to do RNA-seq analysis on a single organism? More specifically, if a person wanted an RNA-seq analysis of expression for a single organ, how much tissue would they need to donate?

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    $\begingroup$ Depends on a lot of parameters? Can you add some more details? $\endgroup$
    – WYSIWYG
    Feb 8, 2017 at 14:23

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Our DNA core wants 1.5 ug of RNA. I find that I can obtain that amount from a standard Trizol prep from about 1 mg of Arabidopsis floral tissue. I usually double or triple what I harvest to account for random variation in yield.

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    $\begingroup$ Any sources to add? +1 for this contribution and welcome 2 Bio $\endgroup$
    – AliceD
    Mar 10, 2017 at 22:28
  • $\begingroup$ ThermoFisher has literally everything in tabular format. As a side note I'd also peg an A260/A280 > 1.8 as equally important as yield for best results. $\endgroup$
    – CKM
    Mar 10, 2017 at 23:21
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http://healthcare.utah.edu/huntsmancancerinstitute/research/shared-resources/center-managed/high-throughput-genomics-site/services/illumina-sequencing/rna-sequencing.php

The Illumina TruSeq RNA Sample Prep Kit v2 with oligo(dT) selection recommends an input of 100 to 1000 ng of total RNA in a volume of 30 ul for library construction.

The Epicentre TotalScript RNA-Seq Kit (Epicentre cat# TSRNA1296) is designed for an input of 1-5 ng of total RNA in a volume of 12 ul to a RNA sequencing library.

Whether you will get a complete picture of a whole organ from such a small slice is an entirely different question. The brain has different regions, with different gene expression.

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