So I'm trying to simulate the production of 3-Hydroxypropionic Acid with E.coli via the Propionyl-CoA Pathway. However I'm not really sure how the production in the pathway goes. I got the following picture: enter image description here

If I understand it correctly the pathway goes: Acetyl-Coa--> Propionate --> Propionyl-CoA --> .... --> 3-HP When adding these reactions in Cobra are the stoichiometric coefficients always just 1?

Source of the figure: Luo H, Zhou D, Liu X, Nie Z, Quiroga- Sánchez DL, Chang Y (2016) Production of 3- Hydroxypropionic Acid via the Propionyl-CoA Pathway Using Recombinant Escherichia coli Strains. PLoS ONE 11(5): e0156286. doi:10.1371/journal. pone.0156286


If I understand it correctly the pathway goes: Acetyl-CoA --> Propionate --> Proprionyl-CoA --> ... --> 3-HP

Not exactly. The first reaction is: $propionate + acetyl\textrm-CoA \rightarrow propionyl\textrm-CoA + acetate$ The CoA group is transferred from acetate to propionate. The following reactions are an oxidation to acryloyl-CoA and a subsequent hydration to 3-HP-CoA. Lastly the CoA group is transferred back to acetate.

Propionate is a 3 carbon molecule and so is 3-HP. So stoechiometric coefficients of 1 seem logical.


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.