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So I'm trying to simulate the production of 3-Hydroxypropionic Acid with E.coli via the Propionyl-CoA Pathway. However I'm not really sure how the production in the pathway goes. I got the following picture: enter image description here

If I understand it correctly the pathway goes: Acetyl-Coa--> Propionate --> Propionyl-CoA --> .... --> 3-HP When adding these reactions in Cobra are the stoichiometric coefficients always just 1?


Source of the figure: Luo H, Zhou D, Liu X, Nie Z, Quiroga- Sánchez DL, Chang Y (2016) Production of 3- Hydroxypropionic Acid via the Propionyl-CoA Pathway Using Recombinant Escherichia coli Strains. PLoS ONE 11(5): e0156286. doi:10.1371/journal. pone.0156286

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If I understand it correctly the pathway goes: Acetyl-CoA --> Propionate --> Proprionyl-CoA --> ... --> 3-HP

Not exactly. The first reaction is: $propionate + acetyl\textrm-CoA \rightarrow propionyl\textrm-CoA + acetate$ The CoA group is transferred from acetate to propionate. The following reactions are an oxidation to acryloyl-CoA and a subsequent hydration to 3-HP-CoA. Lastly the CoA group is transferred back to acetate.

Propionate is a 3 carbon molecule and so is 3-HP. So stoechiometric coefficients of 1 seem logical.

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