So I'm trying to simulate the production of 3-Hydroxypropionic Acid with E.coli via the Propionyl-CoA Pathway. However I'm not really sure how the production in the pathway goes. I got the following picture:
If I understand it correctly the pathway goes: Acetyl-Coa--> Propionate --> Propionyl-CoA --> .... --> 3-HP When adding these reactions in Cobra are the stoichiometric coefficients always just 1?
Source of the figure: Luo H, Zhou D, Liu X, Nie Z, Quiroga- Sánchez DL, Chang Y (2016) Production of 3- Hydroxypropionic Acid via the Propionyl-CoA Pathway Using Recombinant Escherichia coli Strains. PLoS ONE 11(5): e0156286. doi:10.1371/journal. pone.0156286