The images below were obtained in an experiment to enumerate leukocytes in a blood sample using Neubauer counting chamber.
The following solution was prepared and added to the Neubauer chamber and viewed under a compound microscope:
100 microlitres of acetic acid (acidic reagent to haemolyze RBCs) + 50 microlitre of Leishman's stain + 1-2 drops of blood.
Are these granular transparent circular objects in the images the leukocytes? If yes, do these include the smaller ones too? I am unable to decide which ones to take into account.
Moreover is this count normal (for instance, I counted around 250 (excluding the very small ones and applying the margin rule) in the top left box of the grid (Image 3))?