This image is obtained from this paper.
The description of this image is as follows:-
DNA-sequence reads from a tumor sample are aligned to a reference genome (shown in gray). Single-nucleotide differences between reads and the reference genome indicate germline single-nucleotide variants (SNVs; green circles), somatic SNVs (red circles), or sequencing errors (black diamonds). (a) In a pure tumor sample, a location containing mismatches or single nucleotide substitutions in approximately half of the reads covering the location indicates a heterozygous germline SNV or a heterozygous somatic SNV - assuming that there is no copy number aberration at the locus. Algorithms for detecting SNVs distinguish true SNVs from sequencing errors by requiring multiple reads with the same single-letter substitution to be aligned at the position (gray boxes). (b) As tumor purity decreases, the fraction of reads containing somatic mutations decreases: cancerous and normal cells, and the reads originating from each, are shown in blue and orange, respectively. The number of reads reporting a somatic mutation decrease s with tumor purity, diminishing the s ignal to distinguish true somatic mutations from sequencing errors. In this example, only one heterozy gous somatic SNV and one hetererozygous germline SNV are detected (gray boxes) as the mutation in the middle set of aligned r eads is not distinguishable from sequencing errors.
This is my understanding below. Am I correct?
There are multiple reads that are aligned such that for a given location in the reference genome,(here fourth), if approximately half or more of the reads covering that location has a single nucleotide mismatch with the reference genome,then it is a heterozygous SNV.