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For one of our reaction, we require NADPH enzyme. We have NADP in our stock. Is it possible to convert NADP to NADPH using D-glucose anhydrous and if so please provide me how to go about it?

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    $\begingroup$ Reduction potential of NADP⁺ is -0.32 and that of glucuronic acid to glucose is 0.05. So, I guess that glucose oxidation to glucuronic acid cannot reduce NADP⁺. $\endgroup$ – WYSIWYG Apr 1 '17 at 9:15
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    $\begingroup$ @WYSIWYG But glucose may be oxidized (dehydrogenated) to the lactone. glucose 1-dehydrogenase. I think the way this is done is to use hexokinase and NADP-linked glucose-6-phosate dehydrogenase (but you may need to get rid of the ATP). Details not accessible to me at the moment, but perhaps later. $\endgroup$ – user1136 Apr 1 '17 at 14:22
  • $\begingroup$ @tomd you would also need the enzyme. I assumed that OP wants to use the property of reducing sugars to reduce NADP. $\endgroup$ – WYSIWYG Apr 2 '17 at 8:30
  • $\begingroup$ OK. But IMO there is an important principle here. In my experience, the NAD(P)-linked oxidation of an aldehyde to a carboxylic acid is practically irreversible, and it does not matter that this aldehyde is present in minute amounts (as in the open-chain form of glucose). In any event, D-glucuronic acid is reducing (see here. Perhaps you man gluconic acid, the 'mild' oxidation product of D-glucose? $\endgroup$ – user1136 Apr 3 '17 at 16:36

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