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The DNA is read 3'->5' (and RNA synthesized 5'->3'). But due to the DNA strands having to be complementary, it seems to me that the origin can only appear on the correct side of the ORF on one of the strands. And even if it were possible to synthesise RNA from the other strand, due to the directionailty of the strands/RNA the RNA transcript would then code for the protein in reverse. Surely the reverse synthesis of a protein, even if containing all of the same amino acids, would lead to dfferent folding and thus only on eof the proteins is correct?
Now that I am considering the origin, it seems likely to me that the strand on which the origin is dictates which strand acts as the RNA template (perhaps both starnds can, however for a given GENE the RNA is always synthesised from the same strand.
Is this the case? I have read this post: Majority of transcripts are from sense strand? however I found it slightly confusing. I think what the answerer said is similar to what I have suggested here, however I am not quite sure. Would greatly appreciate some input.