I have read a journal that describes sequential passaging with the purpose of measuring resistance development in bacteria. I would like to know more about how the method works ie. what does "passage" mean?

https://www.nature.com/nature/journal/v517/n7535/pdf/nature14098.pdf, Methods part, under resistance studies

  • $\begingroup$ Can you please link the article? $\endgroup$
    – Chris
    May 1 '17 at 11:00
  • $\begingroup$ The word "passage" in biological research methods generally refers to transferring a part of a culture (cell/bacterial/animal) from old medium to new medium. $\endgroup$
    – Armatus
    Nov 18 '17 at 16:19

I am normally reluctant to cite Wikipedia articles, however, the one for Sequential or Serial Passaging explains it quite nicely.

In short:

In microbiology, sequential or serial passaging refers to the process of growing bacteria or viruses in iterations. In essence this means that a virus or a strain of bacteria will be isolated and allowed to grow for a period of time. After the sample has grown for some time, part of it will be transferred to a new environment and allowed to grow for the same period of time.This process will be repeated as many times as desired. The final product is studied, often in comparison with the original virus or bacterial strain to see whether it has evolved or any other changes have occurred.


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