0
$\begingroup$

Do I extract RNA or DNA from gut microbiome (stool samples) if want to do 16s rRNA sequencing?

$\endgroup$
3
  • 2
    $\begingroup$ Genomic DNA, see Illumina's own protocol. $\endgroup$
    – CKM
    May 4, 2017 at 21:07
  • $\begingroup$ More correctly: you are sequencing 16s rDNA, the gene coding for the 16S rRNA. $\endgroup$
    – skymningen
    May 5, 2017 at 10:12
  • $\begingroup$ Well, it depends on what you want to know. If you want to find who is there, then DNA, if you want to know who is there and their functionality, then RNA. Though if you have a lot of samples and limited budget, 16S rRNA is a cheap reliable option. $\endgroup$
    – aLbAc
    Mar 8, 2019 at 15:16

1 Answer 1

3
$\begingroup$

In general, you extract DNA, then PCR out the 16rRNA coding regions and finally sequence them. Here some links

http://press.igsb.anl.gov/earthmicrobiome/protocols-and-standards/16s/

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0068739

https://support.illumina.com/content/dam/illumina-marketing/documents/products/other/16s-metagenomics-faq-1270-2014-003.pdf

$\endgroup$

You must log in to answer this question.

Not the answer you're looking for? Browse other questions tagged .