The article about "rapture sequencing" found here says:

"This protocol also recovers more unique (nonclonal) RAD fragments, which improves both standard RAD and Rapture analysis. Rapture then uses an in-solution capture of chosen RAD tags to target sequencing reads to desired loci. Rapture combines the benefits of both RAD and sequence capture, i.e., very inexpensive and rapid library preparation for many individuals as well as high specificity in the number and location of genomic loci analyzed."

My question is regarding the second sentence. What does it mean to combine in-solution capture of chosen RAD tags to target sequencing reads to desired loci? How do the RAD tags target sequencing reads, if that is what is being said here? How does this relate to combining the benefits of both reduced representation sequencing techniques, or am I missing the crux of the paper?



Schematic overview of the new RAD protocol and RAD capture (Rapture) method. (A) RAD tag isolation. Two wells are depicted in each of two different plates. Genomic DNA is digested with a restriction enzyme and ligated to biotinylated well barcode adaptors (yellow and blue bars). (B) RAD tag isolation and library preparation. DNA from each well is pooled platewise, mechanically sheared, and incubated with streptavidin beads. Following washing, DNA is cleaved from the beads leaving the well barcodes. Finally, a library preparation is performed where a unique plate barcode is added (red and purple bars). (C) Rapture. Multiple plate libraries are pooled, hybridized to biotinylated oligonucleotide baits corresponding to the targeted RAD tag loci, and captured to produce the final library enriched for the loci of interest.

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