I have a really simple question, but I'm not able to figure it out.
I made a tree (using BLOSUM62). Here is a part of the tree:
- just for HBB 36.21 and for HBD 38.79
- or 38.79 + 36.21 = 75
I determined if this set of sequences follows a molecular clock, and indeed this was the case. So I determined the rate at which these sequences envolve. If I can determine the distance between HBD and HBB I can devide this by the rate to obtain the time since the duplication event happened.