Everyone talks about how DNA is the "molecule of life" and "the set of instructions for building you." How complete are the instructions, really, though? To make the question concrete, say I were to remove all of the DNA from a bacterium and replace it with the nuclear DNA from a eukaryote, would the cell (and it's daughters) be able to transition into the same sort of eukaryote as the DNA source? Granted, the lack of mitochondria would be a huge hindrance, but assume we could add some when the bacteria swelled up to a large enough size to accomodate them inside of it. Are there other barriers to this process?
I know that adding any single gene for producing a protein would function as expected when added to a bacterium, but would the lack of a nucleus and organelles prove fatal, or does the nuclear DNA have the information needed to build them up using only the machinery available in a bacterium?
What about an archaea? Or going the other direction - remove the nucleus from a eukaryote, add bacterial DNA, does the cell and its daughters survive to become bacteria?