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Does it make ANY difference to which strand of DNA double helix base pair heads? I mean, could base pair AT and base pair TA makes any difference when interpreted by biochemical mechanisms which use DNA?

Double helix has two strands, right? So does pair Adenine on the first and Thymine on the second strand make any difference with Adenine on the second and Thymine on the first?

I am a novice in biochemistry, so please excuse me my ignorance if the question is ridiculous.

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Absolutely yes, is the short answer.

All genes are encoded in a 5-prime $\rightarrow$ 3-prime manner on each of the 2 DNA strands of the double helix.

Here's an example of a stretch of the genome from a bacteria that I work on in my PhD:

enter image description here

Each of the blue boxes is a separate gene. You can see they're able to be encoded on both strands, and run in opposite directions.

Consequently, whether a base pair is AT or TA changes the overall sequence, and would affect any gene which happens to span that base pair.


When reading the DNA during transcription, the strand that the gene resides on is called the Template (sometimes "Sense") strand.

enter image description here

The strand is separated by RNA polymerase enzymes, and it then uses the opposite strand of the DNA, known as the "anti-sense" strand, to create a copy of the gene sequence, but in RNA instead of DNA (and Thmidine bases are substituted for Uracil, as in the diagram).

Thus, the polymerase creates a mirror image of a mirror image, restoring the sequence of the original strand, but in it's RNA representation.

Flipping the bases round in the helix would change the sequence in the DNA molecule, and this would be propagated by the enzymes etc. which rely on it for transcription and replication.

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