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I know that when two sugar molecules (like glucose) connect to each other, H2O is released because of the -OH and -H groups in both of the molecules. I want to know if the same thing happens when two nucleotides connect to each other during DNA replication.

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    $\begingroup$ Welcome to Biology.SE! What research have you done before asking it here? $\endgroup$ – another 'Homo sapien' Jul 3 '17 at 2:23
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yes there is no water release during phosphodiester linkages because the 3'OH of the growing daughter strand exerts a nucleophilic attack on the phosphodiester linkage between the alpha phosphate with the beta & gamma phoshate of the deoxyribonucleoside triphosphate.

During such formation there is no hydrolysis rather it would precisely be a transesterification type of organic reaction.

REFERENCES:TEXT BOOK OF BIOCHEMISTRY(T.M. DEVLIN) https://en.m.wikipedia.org/wiki/Transesterification

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No, nucleophillic attack by the activated 3'-hydroxyl on the α-phosphate of the incoming nucleoside triphosphate results in the formation of a new phosphodiester bond and release of pyrophosphate (PPi), which is subsequently hydrolyzed.

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[image source]

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