I need to remove any traces of linear DNA (both single and double stranded) from a ligation reaction while keeping circular DNA intact. Up to now, I have used Epicentre's Plasmid Safe to do the job. I would like to try another solution (mostly because of the unreliability of our supplier: it takes weeks to get a batch!).
Has anyone replaced Plasmid Safe with other nucleases? Which one? I have been looking at the NEB nucleases and I was thinking to use ExoV and T together to get rid of any linear DNA (both ssDNA and dsDNA). Does anyone have any other solution to propose?