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I am working now to prepare an overnight bacterial culture. My question is should i pick only one colony of the bacterium streaked plate in order to make an overnight culture and what will happen if i picked two or three colonies?. I am working to make a deletion mutation in one bacterial starin using the phage transduction method. The technqiue said that i should make an overnight culture in 20 ml of the medium. So, i wondered if it would be two low if i pick only one colony in such amount.

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closed as unclear what you're asking by David, Bryan Krause, canadianer, another 'Homo sapien', AliceD Jul 23 '17 at 23:10

Please clarify your specific problem or add additional details to highlight exactly what you need. As it's currently written, it’s hard to tell exactly what you're asking. See the How to Ask page for help clarifying this question. If this question can be reworded to fit the rules in the help center, please edit the question.

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    $\begingroup$ Broadly the answer is 'yes, work from a single colony' but can you give a few more details of your experiment so that the potential pitfalls can be explained? $\endgroup$ – Alan Boyd Jul 20 '17 at 18:12
  • $\begingroup$ I am working to make a delation mutation in one bacterial starin using the phage transduction method. The technqiue said that i should make an overnight culture in 20 ml of the medium. So, i wondered if it would be two low if i pick only one colony in such amount. $\endgroup$ – MIH28 Jul 24 '17 at 8:22

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