If we were to use a Flow cytometry can it seperate either the father or mother chromosomes from a sperm?

  • $\begingroup$ Welcome to Biology.SE. I thought I understood the question but after our discussion in the comments under my answer, I see that I actually don't understand what you mean. Can you please expand your post to clarify your question? In the meantime, I am voting to close as unclear. $\endgroup$ – Remi.b Jul 22 '17 at 23:33
  • $\begingroup$ There are no "mother chromosomes" in a sperm since the sperm comes from the father. If you mean the mother of the sperm donor, there are no "mother chromosomes" because each chromosome is in general a mix of DNA from the father and the mother. $\endgroup$ – mgkrebbs Jul 23 '17 at 1:37
  • $\begingroup$ @mgkrebbs but we have 46 chromosomes from both father and mother right?So the sperm should contain 46 chromosomes can you seperate the another 23 chromosomes from the sperm? $\endgroup$ – Daniel Jul 23 '17 at 9:33

If we were to use a Flow cytometry can it seperate either the xx or xy chromosomes from a sperm

A sperm is haploid. It contains either Y or X but not XY or XX.

Sperm sorting is the process of sorting sperm cells based on specific trait of interest. Whether the sperm is carrying a X or a Y chromosome is definitely of interest to many.

There are a number of methods for sperm sorting (see wiki > sperm sorting > methods), one of which is indeed flow cytometry. Flow cytometry is typically used to seperate X bearing sperms form Y bearing sperms. You should typically have a look at Beltsfield Sperm Sexing Technology (Johson et al. 1999).

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  • $\begingroup$ I'm actually asking can we seperate those xy or xx chromosome that carry genes? $\endgroup$ – Daniel Jul 22 '17 at 22:24
  • $\begingroup$ I mean the autosomes from the father or mother chromosomes $\endgroup$ – Daniel Jul 22 '17 at 23:17
  • $\begingroup$ I don't understand.... Do you mean whether we can separate sperms carrying the grandmother haplotype or the grandfather haplotype? Note that segregation and recombination make that gametes carry some mixture of the two grandparents haplotypes. (Here by grandparent, I refer to the parents of the male producing the sperm). Btw, is there any reason you talk about sperms and not ovules? $\endgroup$ – Remi.b Jul 22 '17 at 23:24
  • $\begingroup$ I asking is it possible to empty all the chromosomes genes from the father or mother from the sperm to have either father or mother chromosomes? $\endgroup$ – Daniel Jul 22 '17 at 23:29
  • $\begingroup$ The expression chromosomes genes is unclear and suggest that you don't really understand what a gene. I am still a bit unsure of what you are asking but you can't tell what chromosome come from which grandparent (whether in a sperm cell, in an ovule or in a zygote), the main reason being that recombination shuffled them up together. $\endgroup$ – Remi.b Jul 22 '17 at 23:32

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