I am analysing RPE-1 cells from humans and I do not understand why I see so many kinetochores by immunofluorescence (more than 100 in many cells). They are in prometaphase.

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  • $\begingroup$ Are you sure that they are RPE-1? Not a cell culture expert but other common cell lines are highly aneuploid (e.g. researchgate.net/post/…), and cell line contamination is very common. $\endgroup$ – Maximilian Press Jul 26 '17 at 4:59

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