3
$\begingroup$

I am analysing RPE-1 cells from humans and I do not understand why I see so many kinetochores by immunofluorescence (more than 100 in many cells). They are in prometaphase.

enter image description here

$\endgroup$
  • $\begingroup$ Are you sure that they are RPE-1? Not a cell culture expert but other common cell lines are highly aneuploid (e.g. researchgate.net/post/…), and cell line contamination is very common. $\endgroup$ – Maximilian Press Jul 26 '17 at 4:59

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.