Suppose a plant cell wants to take something out of its central vacuole. Some specific substance. Well if the vacuole is a bit of a soup of water and other dissolved substances, how does the cell get some specific substance out? Without letting any of the water/solution into the vesicle that's budding off? Or do they just let the solution right in?
Clarification: Since a vacuole is full of water and other dissolved substances, wouldn't that solution get into a budding vesicle? How is this prevented? Is it prevented?
When the cell is creating a vesicle, it starts pinching off part of the membrane, correct? My question is how the solution in the vacuole stays out of the vesicle, if it does at all.