In Uncompetitive inhibitors , the Km decrease . With uncompetitive inhibitors , the affinity of the enzyme for the substrate (E+S) increases .
I've searched up on the net and it says that the substrate has now been taken up to form E-S and E-S-I , therefore consuming more substrate than the original inhibitor-free equilibrium .
E + S -> ES (equilibrium position shifts to the right)
I've read up on all these and more doubts form in me .
Why is substrate needed to form ESI ?
I understood what it meant by equilibrium position will shift to the right . It is harder to form E + P as more substrate will form ESI.