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I need help: I would like to prepare an HEPES solution, without using the powder. It is possible? I remember that I did it once, but maybe I'm wrong...does someone know a protocol for HEPES preparation without using a powder? thanks!!

I need It for morpholino dilutions. 5mM, ph 7.6.

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closed as off-topic by David, canadianer, Bryan Krause, mgkrebbs, CKM Aug 15 '17 at 18:32

  • This question does not appear to be about biology within the scope defined in the help center.
If this question can be reworded to fit the rules in the help center, please edit the question.

  • $\begingroup$ Why don't you want to use the powder? If not, you can only buy stock solutions from the usual providers of lab chemicals. $\endgroup$ – Chris Aug 15 '17 at 12:11
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    $\begingroup$ I'm voting to close this question as off-topic because this is not about biology but about preparing buffer solutions. $\endgroup$ – David Aug 15 '17 at 12:14
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HEPES is a particular chemical, not a mixture of chemicals so unless you have the ability to synthesise your own chemicals you cannot produce it without having the dry chemical preparation.

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