I need help: I would like to prepare an HEPES solution, without using the powder. It is possible? I remember that I did it once, but maybe I'm wrong...does someone know a protocol for HEPES preparation without using a powder? thanks!!

I need It for morpholino dilutions. 5mM, ph 7.6.


closed as off-topic by David, canadianer, Bryan Krause, mgkrebbs, CKM Aug 15 '17 at 18:32

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  • $\begingroup$ Why don't you want to use the powder? If not, you can only buy stock solutions from the usual providers of lab chemicals. $\endgroup$ – Chris Aug 15 '17 at 12:11
  • 2
    $\begingroup$ I'm voting to close this question as off-topic because this is not about biology but about preparing buffer solutions. $\endgroup$ – David Aug 15 '17 at 12:14

HEPES is a particular chemical, not a mixture of chemicals so unless you have the ability to synthesise your own chemicals you cannot produce it without having the dry chemical preparation.


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