I'm interested in the idea of using exosomes as an alternative transfection agent / delivery mechanism for gene editing applications (CRISPR/cas9, etc).

Information on the matter is sparse.. Does anyone know of any practical techniques for manufacturing exosomes in the lab?

  • $\begingroup$ Information on the matter is sparse You can search for any paper that studies exosomes and they'll describe how they obtained them. Is there something specific you cannot find? $\endgroup$ – canadianer Sep 12 '17 at 17:35
  • $\begingroup$ "Manufacturing" in the sense of isolation for research or as a clinical product? Current production methods involve multiple ultracentrifugation, filtration and wash steps followed by detection by immunoassay with culture supernatants as a starting material. In other words, you need an ultracentrifuge. There are small-scale kits for exosome isolation but there's a lot of contaminants often times. Try this publication if you can. Wolters-Kluwer/Ovid are hard to access IMO. $\endgroup$ – CKM Sep 14 '17 at 1:16
  • $\begingroup$ @canadianer isolation is not easy for exosomes of certain cell types like cardiomyocytes, where you'd need direct access to a heart. And many cells often only accept exosomes that are specific to those cell types. In light of this problem, it would be desirable to have a way to manufacture or synthesize exosomes tailor made to certain cell types. That's really what I'm looking for. Techniques for synthesis rather than isolation. $\endgroup$ – WagonWheelWilly Sep 16 '17 at 23:52

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