I am working on a digital holographic microscope. I would like to compare its imaging properties with an actual phase contrast light microscope. My aim is to do this cell by cell, creating a z-stack that shows the same cells in suspension, in a volume with approx. 1-2mm side length. In order to do this, I need to fix each cell firmly in one XYZ position to be able to move them between microscopes.

I am not a trained biologist and I am wondering if there is known procedures/agents to do this. Here's a couple of (non-)requirements I could think of so far:

  • the cells don't need to live through the process
  • they should however retain their form for at least couple of minutes (acquisition time)
  • the agent should be close to translucent and not form bubbles
  • 1
    $\begingroup$ you can embed the cells in a matrix. You can use agarose, geltrex or fibronectin. I am not sure about their optical properties though. $\endgroup$ – alec_djinn Sep 13 '17 at 12:08
  • $\begingroup$ @alec_djinn Thank you. Someone has pointed me to low-melting-point agarose before.. 600 EUR for 50g though, what in the name of God.. $\endgroup$ – smcs Sep 13 '17 at 12:11
  • 1
    $\begingroup$ You can use normal agarose as well, it is much cheaper. A 0.5% will do the job. $\endgroup$ – alec_djinn Sep 13 '17 at 12:13

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.