I am working on a digital holographic microscope. I would like to compare its imaging properties with an actual phase contrast light microscope. My aim is to do this cell by cell, creating a z-stack that shows the same cells in suspension, in a volume with approx. 1-2mm side length. In order to do this, I need to fix each cell firmly in one XYZ position to be able to move them between microscopes.
I am not a trained biologist and I am wondering if there is known procedures/agents to do this. Here's a couple of (non-)requirements I could think of so far:
- the cells don't need to live through the process
- they should however retain their form for at least couple of minutes (acquisition time)
- the agent should be close to translucent and not form bubbles