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I am trying to study/learn metagenomics through 16s rRNA microbial data and I want to teach myself/review core biology concepts while also learning the QIIME 2 analysis pipeline, as the website suggests learning it instead of QIIME for beginners.

I found a great source : http://www.science.smith.edu/cmbs/wp-content/uploads/sites/36/2015/09/Tutorial-from-sample-to-analyzed-data-using-Qiime-for-analysis.pdf but was hoping for something with an analysis I could actually work through on my own, as this one involves the use of a cluster, where as I only have access to a laptop of moderate power and also one that involves QIIME 2.

Also something free and maybe as hand-holding as possible would be ideal too.

As for the platform I am learning on, it is a virtual box instance of Ubuntu.

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The QIIME 2 tutorials are the place to get started - see here.

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  • $\begingroup$ So do you think those tutorials could take someone from layman to decent? I read on your profile that you teach bioinformatics so I am willing to take any other advice too. Thanks $\endgroup$ – Ro Siv Sep 21 '17 at 18:20
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    $\begingroup$ They will teach you QIIME 2 without assuming much background knowledge. If you're new to the idea of studying microbiomes, you'll probably want some other resources too, possibly including Gut Check and An Introduction to Applied Bioinformatics. (Disclaimer: I'm involved in both of those projects, but all content is free.) $\endgroup$ – gregcaporaso Sep 21 '17 at 22:13

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