-1
$\begingroup$

Is there any way to calculate the number of bands formed due to sodium dodecyl sulphate-polyacrylamide gel electrophoresis for a particular compound of group of proteins? For example, how could I calculate the number of bad formed after electrophoresis of IgG (immunoglobulin G) under oxidizing or reducing conditions?

$\endgroup$
  • $\begingroup$ This sounds like a homework question, which are not allowed unless you show your attempt at the answer. Do you know what reducing conditions will do to a protein? Do you know the structure of immunoglobulins? $\endgroup$ – canadianer Oct 7 '17 at 15:35
0
$\begingroup$

Okay, so I think I figured out what was required. Immunoglobulins have 2 light chains and 2 heavy chains. So 2 distinct bands would be formed (the heavier one at the back and the lighter one in the front) I suppose.

$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.