I am one of the biotech students. I want to know the dynamic ranges of interleukin 2 and 12 that i can use to activate the NK-92 Cells. thank you
If you have the resources you should ideally titrate your cytokines. You'll find that cytokines from different vendors may exhibit varying stability or availability in vitro, but also that you obtain varying levels of activation. Standard NK-92 culture conditions are 100-300 IU/mL IL-2 in RPMI1640 medium, 10% serum and 2mM L-glutamine (or 1X GlutaMAX, life technologies). Activation levels depend on how much you want to activate them. Interleukin-12 I'm fairly certain is standard at 10 ng/mL, but should also be titrated.
There's actually a relevant paper with a fair titration looking at granzyme expression at different concentrations of IL2, IL12 and IL15, and different timepoints. They note that NK92 cells show response at 100, 500 and 1000 IU/mL IL2, and at 10-30ng IL12.